Development on DNA detection kit of fox and its application in common meat products

OBJECTIVE: To develop a kind of DNA extraction and detection kit for identification of fox source composition. METHODS: Using the modern DNA fingerprint technology, the DNA extraction method was improved, and DNA extraction and detection reagent was developed to obtain the fox polymerase chain reaction( PCR)detection kit. The performance parameters of the kit were evaluated. Finally, 42 samples of fox meat and its mixture with commercial meat products were detected. RESULTS: The kit was proved effective after 20 times of the repeated frozen-thaw and it could be stored at-20 ℃ for 1 year. The specificity test confirmed that fox source composition were detected from 42 samples of fox meat and its mixture with commercial meat. The specificity of the kit was 100%. The minimum detection limit of DNA was 0. 1 ng/μL. CONCLUSION: The fox DNA detection kit could be applied in rapid detection commonmeat of fox source composition, which are good specificity, high sensitivity and good stability.