Sialyltransferase-mediated glycoPEGylation

Abstract GlycoPEGylation is a site-selective PEGylation method developed for modifying complex glycoproteins. It relies on the ability of glycosyltransferases to efficiently transfer polyethylene glycol (PEG)–modified sugar nucleotides to the glycan termini of glycoproteins. GlycoPEGylation is performed at neutral pH and modest temperature, and it is therefore well-suited for PEGylation of chemically sensitive proteins such as coagulation factors. In this chapter, sialyltransferase-based glycoPEGylation technology and its application for half-life extension of cytokines and coagulation factor proteins is described.