Preparation method for thymosin [alpha]1 and analogues thereof

The invention discloses a solid phase synthesis and purification process for thymosin [alpha]1 and analogues thereof, and belongs to the technical field of peptide synthesis. The process comprises the following steps of 1) taking a tricyclic amide linker resin as a carrier; 2) connecting side chain carboxyl groups of Fmoc-Asp-Y to amino groups of the resin after the resin is subjected to deprotection of Fmoc; 3) synthesizing residual amino groups in order in a solid phase by using a Fmoc strategy; 4) removing Fmoc protection groups from N-terminal amino protection groups, acetylating the N-terminal amino protection groups by using a DMF solution of Ac2O and DIPEA; 5) cutting by using a lysis reagent to obtain a crude peptide and precipitating the crude peptide with ether; and 6) separating the crude peptide by HPLC to obtain a pure product.