Evaluation of “in house” criteria for PCR-based analysis in immigration casework

Abstract “In house” criteria for PCR-based analysis in immigration casework have been evaluated in 28 immigration families. The isolated DNA was amplified using the multiplex systems SGM+ (Applied Biosystems), CTTv, FFFL and PowerPlex 16 (Promega). PCR products were separated on the ABI 310 analyzer. The one and two parental indices ( I ) were calculated using the DNA.VIEW software package (C. Brenner, Oakland, USA). DNA was isolated twice to reduce mistakes. Combining the results of the multiplex STR systems, used in this evaluation, appeared to be successful in analysing immigration casework. From the results of this evaluation, it appeared that the involvement of other family members in immigration casework has to be critically evaluated.