Synthesis, biological evaluation and docking study of possible antifungal compounds with a coumarin-containing triazole side chain

Abstract. Due to increasing drug resistance by Candida species, especially in hospitals, the search for new antifungal agents has intensified. The incorporation of the coumarin scaffold into several nitrogen-containing heterocyclic moieties reportedly increases antimicrobial efficiency. The aim of this study was to design and synthesize a series of simple coumarin-linked triazole derivatives and test their possible antifungal activity against four Candida species. Docking simulations were conducted to explore the binding properties of the test compounds and compare them to reported data on fluconazole, the reference drug. Starting from 3-acetylcoumarins, coumarins 2a-d, 3a-c and 4a-d were obtained in high yields. The concentration of each compound needed to inhibit the Candida species was determined by serial dilution. An inhibition of 62% of C. albicans was produced by 2b (300 µg/ml), 87% of C. tropicalis by 3a (100 µg/ml), 89% of C. parapsilosis by 3a (500 µg/ml), and 87% of C. glabrata by 4a (300 µg/ml). The values ​​of antifungal activity were similar for the coumarin derivatives and fluconazole, the latter of which induced 90% inhibition of the four yeasts at 500 µg/ml. According to the docking simulations, the interactions at the active site of the lanosterol 1,4-demethylase enzyme (CYP51) are similar for the test compounds and fluconazole. The subcellular location of the derivatives was identified as the mitochondrion. These coumarins are characterized by structural simplicity, with the simplest structures showing better antifungal activity than fluconazole. Further research is needed to isolate CYP51 and directly test its inhibition by coumarin derivatives.                                                Resumen. Una serie de moleculas de cumarina-triazol se sintetizaron y evaluaron contra diferentes especies de Candida. Las cumarinas 2a-d, 3a-c y 4a-d se obtuvieron utilizando como material de partida las 3-acetilcumarinas en altos rendimientos. La concentracion necesaria de las moleculas para mostrar actividad antifungica contra las cuatro especies de Candida se determino mediante un metodo de diluciones seriadas. Se reporta un 62% de inhibicion de C. albicans usando 2b (300 µg/ml), 87% de inhibicion contra C. parapsilosis por 3a (500 µg/ml), y un 87% de inhibicion a C. glabrata por 4a (300 µg/ml). El efecto de las cumarinas es comparado con el farmaco de referencia fluconazol, que induce un 90% de inhibicion en todas las cepas usando 500 µg/ml. Los resultados del estudio Docking muestran que las interacciones de todas las moleculas en el sitio activo de la enzima CYP51 son similares a las interacciones presentadas por el fluconazol. Finalmente, tomando ventaja de las propiedades fluorescentes de las cumarinas, la localizacion subcelular y penetracion de los compuestos, fue localizada en las mitocondrias. Las cumarinas reportadas, ademas de presentar sencillez estructural, tambien presentan valores de inhibicion de las cepas comparables, y en los casos mencionados, mejores que el farmaco de referencia.