Identification and expression profiling of neuropeptides and neuropeptide receptor genes in Atrijuglans hetaohei

Abstract Atrijuglans hetaohei Yang (Lepidoptera: Gelechioidea), is one of the major pests that can seriously damage the walnut fruits. Neuropeptides and their receptors regulate most physiological functions in insects and represent new targets for the development of control agents. To identify the neuropeptides and their receptors from A. hetaohei, we sequenced and analyzed its head transcriptomic data, identified 32 neuropeptides and 39 neuropeptide receptor genes. Sequence comparisons and phylogenetic analyses suggest that A. hetaohei neuropeptides and receptor genes have high homology with those in Bombyx mori, Chilo suppressalis, Plutella xylostella and Helicoverpa armigera. Moreover, gene expression patterns revealed that neuropeptide genes such as AKH1, CP, MS and PTTH were expressed specifically in male head, while CAP3, DH, NPLP1, PBAN and SIF showed higher expression in the female head. Bur showed abdomen biased expression in both male and female. Neuropeptide receptor genes such as A8, A11, A15 and LGR were highly expressed in male head, whereas A24 and LGR2 were preferentially expressed in female head. This is the first sequencing, identification and expression analyses of neuropeptides and neuropeptide receptor genes from A. hetaohei. Our results could provide a powerful background that will facilitate the further investigations using transcriptomics to determine neuropeptides and their receptors presence, functions, and indicates potential targets in A. hetaohei for a novel pest management strategy.