Quantitative research on toxic component-wilforlide A in Xinfeng capsules before and after compatibility

Objective To study the content changes of wilforlide A in Xinfeng capsules before and after compatibility. Methods The content of wilforlide A was determined in Xinfeng capsules ester content and in Tripterygium wilfordii Hook.f.single solution by TLCS method. Developing solvents: chloroform and methanol(85:1), scanning wavelength:230 nm. Results Wilforlide A showed a good linear relationship in 1.700- 5.100μg, the regression equation is Y= 1 118.766+ 3 253.751X(r= 0.999). The average recovery of the wilforlide A in Xinfeng capsules was 102.84%, and RSD was 3.64%. The average recovery of the wilforlide A in the Tripterygium wilfordii Hook.f.single decoction was 98.14%, and RSD was 2.21%, compatibility levels before and after the change(compatibility with 0. 179mg/g,0.136mg/g after compatibility). Conclusion The established TLCS method was simple, specific and accurately quantitative. The comparative study found that the compatibility of Xinfeng capsules Tripterygium reduced the content of wilforlide A, which may be related to decreasing toxicity. Key words: Xinfeng Capsules; Wilforlide A