Purification and use of pertactin outer membrane protein of Bordetella pertussis.

RECOVER pertactin (FORMERLY PROTEIN 69 KDA) in a form biologically pure STABLE no activity detectable adenylate cyclase FROM fermentation broth Bordetella pertussis WELL AS from cells. IS THE SOUP TO EXTRACT Selectively pertussis toxin (PT) and filamentous hemagglutinin (FHA), pertactin Rushes WITH AMMONIUM SULFATE AND precipitate is dissolved in buffer at a pH of 0.6 to 8.5, A CONTINUING It misses the solution through HIDROXIAPATITA AND COLUMNS chromatographic Q-Sepharose BEFORE FINAL ultrafiltration. SE EXTRACT cells with UREA AND EXTRACT Ultrafiltration and diafiltration. Pertactin is precipitated from the extract and processed precipitate as described above. In one variation, is contacted the broth with ammonium sulphate to precipitate the pertactin, PT AND HAF is dissolved precipitate and Selectively EXTRACT AND HAF TP before passing the DISOLUCIONA COLUMNS Chromatographic.