Liposomes for DNA Nanotechnology: Preparation, Properties, and Applications

Over the past two decades, DNA has become a major player in nanotechnology. A very interesting and useful method uses DNA to link various nanoparticles, where the programmable structure and molecular recognition function of DNA are coupled to the optical, electric, magnetic, and catalytic property of the nanomaterials. Compared to many inorganic nanoparticles, liposomes are self-assembled soft matters that possess surface fluidity and the potential for molecular containment. The charge, size, and phase transition properties of liposomes can be precisely tuned by varying liposome formulation. In this chapter, we describe methods for liposome preparation and DNA attachment. We also discuss the biophysical properties of DNA-functionalized liposomes and their emerging applications in DNA-directed assembly, biosensor development, and drug delivery.