Характеристика клеточного и биохимического профиля индуцированной мокроты и крови у курящих и некурящих здоровых людей

The aim of this study was to search cell structure of induced sputum (IS), activity of tripsin-like proteinase, elastase and their inhibitors: α 1 -proteinase inhibitor, α 2 -macroglobulin and acid-stable inhibitors in the IS and blood serum and of blood neutrophil activity in smokers and healthy nonsmokers. We examined 67 healthy volunteers aged 19 to 42 yrs. The IS cytograms showed that the absolute amount of neutrophils and the percentage of lym phocytes were significantly higher and the percentage of macrophages was lower in smokers. They also had greater tripsin-like proteinase activity in serum and IS. The average cytochemical coefficient and the percentage of neutrophils with myeloperoxidase granules did not differ reliably in smokers and nonsmokers. There were quite strong positive correlations between smoking, neutrophils, lymphocytes, macrophages in IS and the tripsin-like activity in sputum and serum.