Evaluation of Microbial Load, Formation of Odorous Metabolites and Lipid Stability during Wet Preservation of Nannochloropsis gaditana Concentrates

Wet preservation of algae allows us to bridge the time period between algae harvest and processing while avoiding the costs and nutritional losses associated with algae drying. This study aimed to identify suitable storage conditions for the wet preservation of Nannochloropsis gaditana concentrates. The impact of storage temperature, time and the way of closing the storage recipient was evaluated using a full factorial design. The effect of acetic acid addition was tested for one storage condition. Storage temperature was the main factor determining the microbial count and had a vast impact on the formation of odorous metabolites. Storage at 20 °C in closed recipients led to rapid O2 consumption, accumulation of malodorous short-chain fatty acids above their odor thresholds, and the production of H2S and methanethiol. These odorous metabolites were not formed or to a much lower extent during 4 °C and 8 °C storage in closed recipients. Acetic acid supplementation (50 mM) suppressed the formation of short-chain fatty acids during 8 °C storage in unsealed recipients and reduced the aerobic microbial count and the number of yeasts and molds by approximately one log unit after 14 days. Yet, acetic acid addition also induced lipid hydrolysis and decreased chlorophyll levels when algae were stored for more than one week. This study demonstrated that temperature control is needed and that acetic acid addition is a promising approach when N. gaditana concentrates are stored for less than one week.