The effects of Newcastle disease virus on human leukemia in vitro

Objective To study the effects of Newcastle disease virus (NDV) on human leukemia in vitro. Methods The density and morphologic change of cells infected by NDV was observed. MTT assay was used to investigate the effect of NDV on the proliferation of the leukemia cells. Results The cells of the control were all regular and coarctate by inverted microscope. The effect of NDV were conspicuous in that the cells were irregular, sparsate, aggregate and fused in the experimental groups. MTT assay showed that the proliferation of SHI-1 cells were significantly inhibited by NDV with different concentration (P ＜0.01). The results also demonstrated that the difference within the concentration and the time were both statistically significant (P＜0.01). The inhibition of NDV was further confirmed in a time-concentration-dependent manner.Conclusion Newcastle disease virus can inhibit human acute monocytic leukemic cell proliferation in vitro.The safety of NDV is reliable. Key words: Newcastle disease virus;  Leukemic, monocytic, acute