[Change and clinical significance of peripheral blood T-lymphocyte functional subsets in acute graft-versus-host disease].

Objective:This study was aimed to detect the change of T-lymphocyte functional subsets marked by CCR7 and CD45RA in the aGVHD within 100 days after allo-HSCT and to explore its clinical significance.Methods:The peripheral blood of 42 patients after allo-HSCT was collected every two weeks since hematopoietic reconstitution.The expression of CD3,CD4,CD8,CCR7 and CD45RA-marked T4 ymphocytes was detected by flow cytometry,the relationship between their expression and the prognosis of aGVHD was analyzed.Results:The percentage and the absolute count of CCR7~+ T lymphocyte were significantly reduced in aGVHD.The percentage of T_(naive),T_(CM),T_(EM) and the absolute count of T_(naive),T_(EM),T_(TD) were sharply reduced in aGVHD,moreover has changed correspondingly with outcome of aGVHD.The percentage of CD3,CD4,CD8-mrked T-lymphocyte subsets did not significantly changed.Conclusion:T-lymphocyte functional subsets marked by CCR7 and CD45 RA are a valuable indicator to monitor early immune reconstruction for patients with the aGVHD after allo-HSCT.