Aethusifolins A–D: Four new components from a traditional Mongolian medicinal herb Clematis aethusifolia Turcz

Abstract Four new components named aethusifolins A–D ( 1 – 4 ), together with ten known ( 5 – 14 ) were isolated from the dried aerial parts of a traditional Mongolian medicinal herb Clematis aethusifolia Turcz. The planar structures were established based on extensive spectroscopic analysis (HRMS, 1D and 2D NMR), and the absolute configurations were determined by modified Mosher’s method, hydrolysis method. The cytotoxicities of isolated compounds against a panel of five human solid tumor cell lines were assayed. Compounds 5 , 8 , and 13 showed moderate cytotoxicity against A-375 with IC 50 values of 15–18 μM, while compound 8 also showed cytotoxicity against SH-SY5Y with IC 50 values of 20 μM.