Pulsed field gel electrophoresis : a practical approach

1.: Theories of gel electrophoresis of high molecular weight DNA. 2.: PFGE in physical mapping. 3.: Mutation detection and diagnosis using PFGE. 4.: Cloning from PFGE-purified genomic DNA fragments and YACs. 5.: Yeast artificial chromosome cloning using PFGE. 6.: PFGE analysis of yeast artificial chromosomes. 7.: Functional analysis of mammalian genomes using yeast artificial chromosomes. 8.: PFGE in the study of a bacterial pathogen (Haemophilus influenzae). 9.: Analysis of the genomes of protozoan parasites using PFGE