Preparation of a sulfated linear (1→4)-β-d-galactan with variable degrees of sulfation

Abstract A linear 1,4-β- d -galactan has been prepared by partial acid hydrolysis of the major lupin seed polysaccharide on a preparative scale. The galactan has been characterized by acetylation and methylation analysis, by GPC, as well as by its 1 H- and 13 C-NMR-spectra. Sulfation with increasing amounts of SO 3 -pyridine yielded a series of galactan sulfates with increasing DS. In all derivatives, the sulfate groups had a similar pattern of distribution, as shown by methylation analysis. Beside unsulfated parts of the molecule, there were also parts with a multiple substitution pattern.