Effects of Submaximal Endurance Training and Vitamin D3 Supplementation on Pain Threshold in Diabetic Rats

Article history: Received: 13 Apr 2012 Accepted: 22 Oct 2012 Available online: 7 Jan 2013 ZJRMS 2013; 15(7): 22-25 Background: 2TAccording to beneficial effects2T of endurance training and vitamin DR3R in diabetes mellitus, purpose of this study is effects submaximal endurance training and vitamin DR3 Rsupplementation on pain threshold in streptozotocin induced diabetic rats. Materials and Methods: Male Wistar rats (250±20 g, N=40) were made diabetic by streptozotocin (60 mg/kg, subcutaneously). 72 h after injection diabetes induction was confirmed by tail vein blood glucose concentration (>300 mg/dl). Then animals were divided to five groups: diabetic control (DC), diabetic trained (DT), diabetic -vitamin D (DD), diabetic trained and vitamin D (DTD), and control (C). Animals were submitted to endurance training by treadmill and vitamin DR3R treatment (twice aweek, intrapretonally) for 4 weeks. 48 h after at the end of exercise and treatment protocol, we used tail-flick to assess the effects of training and vitamin DR3R on thermal pain threshold. We used 4Tone way 2T4TANOVA2T 2Tstatistical analysis2T4T 2T4Tto compare 2T4T 2T4Tdifferences between2T4T 2T4Tgroups, 2T4T 2T4Tsignificance2T4T 2T4Tlevel of2T4T p<0.05 2T4Twas considered2T4T. Results: Diabetic induced hyperalgesia were decreased significantly by vitamin D but not 4 weeks endurance exercise training. Concurrent effects of training and vitamin D on thermal pain threshold were not significantly higher than vitamin D effects alone. Conclusion: It is concluded that vitamin D administration given at the time of diabetes induction may be able to restore thermal hyperalgesia. But effects of endurance exercise training needs to more investigation in diabetic rats. Copyright © 2013 Zahedan University of Medical Sciences. All rights reserved.