The method for rapid detection of soybean oligosaccharide content by HPLC

Established a high-performance liquid chromatography method for detecting the content of sucrose、 raffinose and stachyose in soybean grains.Use refractive,index detector with range 0.5,Kromasil-NH2 column imported from Sweden,and its model is 4.6 mm×250 mm 5 μm,the velocity of flow is 1 mL/min,the detection temperature of column is 35 ℃,and the acetonitrile:water=60:40.Under the conditions of the above-mentioned test,the minimum detection limit of source is 0.06×10-6 g,the minimum detection limit of raffinose is 0.07×10-6 g,and stachyose is 0.06×10-6 g.The correlation coefficients of standard curves were 0.9965,0.9996 and 0.9994.The average recoveries were 99.3%,94.7% and 95.6%.The RSD of samples with 10 tests are 1.73%,2.01% and 0.80%.The method is simple,rapid,accurate,and can be used in the oligosaccharide content in soybean quick analysis.