Mapping of the glucoamylase gene of Trichosporon adeninovorans by mitotic haploidization using hybrids from protoplast fusions

In two strains of Trichosporon adeninovorans genes of unknown location were assigned to linkage groups. Diploid strains were produced by protoplast fusion and partially haploidized by growth on benomyl causing mitotic haploidization. We show that both strains synthesize an extracellular glucoamylase with different properties. These differences could be used to map the corresponding gene in the segregants. We could map the glucoamylase gene from one of these yeast strains on linkage group II, one of the three linkage groups identified so far.