Visual identification of individual transfected cells for electrophysiology using antibody-coated beads.
1994
Electrophysiological study of transiently transfected cells requires the identification of individual cells that express the protein of interest. We describe a simple, quick and inexpensive method for visually identifying cells that have been co-transfected with an expression plasmid for a lymphocyte surface antigen (CD8-α). Transfected cells are incubated briefly with polystyrene microspheres (4.5 μm diameter) that have been precoated with antibody to CD8
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