Glycogen synthesis by a strain of ehrlich ascites tumor

Summary A strain of Ehrlich Ascites cells, H, contains glycogen and synthesizes glycogen in vitro from added glucose or fructose. Only a fraction of the cells contain glycogen, and it is distributed in cytoplasm and nucleus. The metabolism of glucose and activities of enzymes of glycogen metabolism in the H cells, was compared to a strain (HL) which does not contain or form glycogen. The activities of glycogen phosphorylase, glycogen synthase, phosphoglucomutase and UDP glucose pyrophosphorylase were of similar magnitude in both strains. Glucogon, epinephrine, and dibutyryl CAMP had no effect on the metabolism of glucose and its incorporation into glycogen in H cells, and were also without effect on its catabolism.