Effect of total saponin of stibmata maydis on carbohydrate tolerance reducing mice model induced by Dexamethasone and α-glucosidase activity in vitro.

Objective:To explore the mechanism and characteristics of total saponin of stibmata maydis in decreasing blood glucose by study the effect total saponin of stibmata maydis on carbohydrate tolerance reducing mice model and α-glucosidase activity in vitro.Methods:The model was established by intramuscular injecting Dexamethasone.Fasting blood sugar and serum blood sugar level were measured to observe the effect of total saponin of stibmata maydis.An enzyme-inhibitor model was established to study the effect of total saponin of stibmata maydis on α-glucosidase activity.Results:Compared with model group,total saponin of stibmata maydis of middle dosage can remarkably reduce blood sugar level after irrigating the glucose for 30min,60min and 20min;high and low dose of total saponin of stibmata maydis and the group of Diamicron can obviously reduce the blood glucose level at every time period;total saponin of stibmata maydis can inhibit the activity of α-glucosidase in vitro and have obvious dose-effect relationship.Fifty percent of α-glucosidase activity is inhibited at 45.07μl(20mg/ml).Conclusion:Total saponin of stibmata maydis had the remarkable function of improving carbohydrate tolerance on reducing mice model induced by Dexamethasone and inhibits α-glucosidase activity.