PENGARUH PENAMBAHAN METIL JASMONAT PADA KULTURKALUS DAUN SELASIH (Ocimum basilicum L. forma violaceum Back.)SERTA UJI BIOAUTOGRAFI TERHADAP Escherichia coli DANStaphylococcus aureus

Basil (Ocimum basilicum L. forma violaceum Back) can be grown in subtropical to tropical regions, including Indonesia. Wide spread of the discrepancies compounds contained therein. It is necessary to in vitro cultivation techniques with callus formation and induction of secondary metabolites using elicitor. Based on these descriptions, research is needed to determine the effect of methyl jasmonate (MeJA) and antibacterial activity against Escherichia coli and Staphylococcus aureus in bioautography. Sterilized basil leaves grown on sterile Murashige-Skoog solid medium with 1 ppm 2,4-D. 3 weeks old callus subculture was transferred to liquid MS medium with 2,4 -D 0.1 ppm control and treatment MeJA (10 mM, 20 mM and 30 mM) for 3 days. Callus growth parameters measured by the increase in wet weight, dry weight and drying shrinkage. The observation cell was conducted to determine the effect of the callus cell in the MeJA. Effect of the addition of MeJA analyzed by Thin Layer Chromatography (TLC) qualitatively. Bioautography test is performed to determine which compound is responsible to the antibacterial activity. Callus control has the highest percentage of dry weight, then progressively decrease with increased concentrations of MeJA. Observations showed that the MeJA in culture can affect the process of mitotic cells with diverse responses and inhibits cell cytokinesis. TLC analysis showed similarities to control callus TLC profile of plant origin. Some compounds are not formed on the callus treatment allegedly caused by the inhibition of the MeJA. Based on bioautography test, ethanol extract of control callus has antibacterial activity against E. coli and S. aureus with estimates of a class of compounds that are responsible is terpenoids.