Biosensors Utilizing Consecutive and Parallel Substrates Conversion
2021
Coupling different enzymes either in sequence or in competition pathway is a way to enhance the range of analyte species accessible to measurement, the selectivity and the sensitivity of biosensors. In this chapter, mathematical models of several types of amperometric multi-enzyme biosensors utilizing consecutive or parallel substrates conversion are modeled and analysed at stationary and transient conditions. A biosensor based on bienzyme electrode with co-immobilized D-glucose oxidase and peroxidase is considered under stationary conditions at excess concentrations of oxygen and ferrocyanide. A trienzyme biosensor utilizing consecutive substrates conversion with three enzymes is modeled at internal diffusion limitation. A biosensor with dual catalase-peroxidase bioelectrode is mathematically modeled by nonlinear reaction–diffusion equations. Finally, multi-enzyme biosensors utilizing parallel and competitive multi-substrate conversion are analysed.
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