Peptide TQS169 prevents osteoporosis in rats by enhancing osteogenic differentiation and calcium absorption

Abstract The preventive effects of TQS169 peptide on osteoporosis were investigated and the mechanisms of promoting osteogenic differentiation and calcium absorption were also studied in vitro and vivo in this research. Wistar rats were given a low-Calcium diet treated with different doses of peptide TQS169 and extra CaCl 2 for 4 weeks. The content of Calcium, OPG in serum and the Ca content of the femur were increased in high-dose-treated rats. The osteoporosis model induced by retinoic acid was established to measure the related factors. High dosage of TQS169 treated group showed the protective effects. Osteoblastogenesis differentiation of MC3T3-E1 cells treated with TQS169 were accelerated through improving ALP activity, promoting mineral matrix formation and the expression of typical genes of RUNX2, OCN and COL I. Taking all the experiments together, our study confirms the protective effects of TQS169 on osteoporosis and explains the potential mechanism for the first time.