Transcardiac 8-iso-prostaglandin F2 αgeneration from acute myocardial infarction heart: insight into abrupt reperfusion and oxidant stress

Abstract 8-iso-prostaglandin F 2 α (8-iso-PGF 2 α ), a representative isoprostane, has been reported to be a reliable marker for oxidant stress in vivo. To examine if 8-iso-PGF 2 α is generated in patients with acute myocardial infarction (AMI), we measured the level of immunoreactive 8-iso PGF 2 α in the great cardiac vein as well as classical eicosanoids, 6-keto-prostaglandin F 1 α (6-keto-PGF 1 α ) and thromboxane B 2 (TXB 2 ) in the process of urgent coronary balloon angioplasty. Fourteen patients with anterior AMI were divided into two groups: the totally occluded ( n =7) and the already perfused groups ( n =7). In the former, transient elevation of 8-iso-PGF 2 α was observed immediately after the angioplasty, i.e. the ratio of post-angioplasty level to pre-level was approximately 2.4 for 8-iso-PGF 2 α , 14 for 6-keto-PGF 1 α , and 5 for TXB 2 . In the already perfused group, the levels of these eicosanoids were unchanged. In the totally occluded group, peak creatine phosphokinase in a peripheral vein was correlated with the level of 8-iso-PGF 2 α (r 2 =0.841, P 2 α generation is a reliable marker for the size of myocardium exposed to oxidant stress.