Temporary augmentation of glycosaminoglycans content in the heart after left coronary artery ligation

Introduction: Augmentation of glycosaminoglycans (GAG) in various tissues after injury is well known, however, there is no information about the metabolism of GAG during the heart remodeling after infarction. The study is focused on the changes of total GAG concentrations in the viable myocardium and scar after experimental left coronary artery occlusion. To shed some light on the possible mechanism of the changes, GAG were also evaluated in the skin. Methods: Male Wistar rats were subjected to left coronary artery ligation or to sham operation. After 3, 6 or 12 weeks of follow up the rats were sacrificed and the heart and skin were collected. The heart was cut into parts: right ventricle, septum, viable region of left ventricle and scar. The Farndale method was used for the estimation of GAG in the samples. Results: High level of GAG in the myocardial scar tissue was seen in the 3 weeks of follow up and reached maximum in the 6 weeks and then decreased in week 12. Similar pattern of GAG changes was found in the contractile part of the heart. In both viable part of the left ventricle and septum the peak level of GAG was found in rats 6 weeks after the onset of infarction. Than the content of GAG decreased towards the control level. There was no alteration in the GAG content in the skin and a wall of the right ventricle. Conclusion: Temporary augmentation of GAG content is present not only in myocardium directly injured by ischaemia but also in the viable part of the heart subjected mainly to increased haemodynamic stresses. The local nature of mechanisms responsible for the GAG changes has been postulated.