Muscle Glyceraldehydephosphate Dehydrogenase: NAD+ Binding and its Implications for the Mechanism of Action of the Enzyme

The binding of NAD+ to rabbit-muscle glyceraldehydephosphate dehydrogenase (D-glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12) has been studied extensively by several groups (1, 2, 3, 4). Since, however, a start has been made with determining the tertiary and quaternary structure of the lobster-muscle enzyme (5) by X-ray crystallographic data, and its primary structure has been elucidated (6), it was important to extend our binding studies to this enzyme (7).