Preparation method of gene chip responding Xanthomonas oryzae, gene chip and application of gene chip

The invention relates to a preparation method of a gene chip responding Xanthomonas oryzae, the gene chip and application of the gene chip. The preparation method comprises the following steps: storing homogenized cNDA (deoxyribonucleic acid) plasmids in a cDNA library and cNDA plasmids in a subtractive cNDA library in holes of a 384 pore plate and successively numbering; and successively extracting the homogenized cNDA (deoxyribonucleic acid) plasmids in the cDNA library and the cNDA plasmids in the subtractive cNDA library according to the number based on SSC as negative control, cDNA of beta-actin as positive control, and preparing the negative control and positive control into the gene chip on a vector. The cDNA type in the gene chip can comprehensively reflect the gene cDNA related to rice bacterial blight resistance so that detection and screening of relevant bacterial blight resistant genes in the subsequent application can be ensured to the greatest extent; and the xDNA average degree of the gene chip is 500bp, and the gene chip has better specificity, thereby improving detection and utilization efficiency.