BHQ-Cyanine Based “Off-On” Long-Circulating Assembly as Ferroptosis Amplifier for Cancer Treatment: a Lipid-Peroxidation Burst Device

Ferroptosis is an iron-dependent cell death caused by accumulation of lipid peroxidation (LPO), which was a new strategy for cancer treatment. Current ferroptosis therapy nano-devices with low efficiency and side effect generally. Hence, we developed a black hole quencher (BHQ) based fluorescence “off-on” nano photosensitizer complex assembly (CSO-BHQ-IR780-Hex/MIONPs/Sor). Chitosan oligosaccharide (CSO) connected BHQ-IR780-Hex and loaded magnetic iron oxide nanoparticles (MIONPs) and sorafenib (Sor) formed a very concise functionalized delivery system. CSO-BHQ-IR780-Hex disassembled by GSH attack, and released IR780-Hex, MIONPs and sorafenib. IR780-Hex anchored to mitochondrial membrane, which would contribute to amplify the efficiency of photosensitizer. When gives NIR irradiation to CSO-BHQ-IR780-Hex/MIONPs/Sor treated cells, iron supply increased, xCT/GSH/GPX-4 system was triggered and lot of LPO burst. Malondialdehyde (MDA) test showed that LPO in complex assembly treated cells was explosive increase...