Effects of Women's Age and Oocyte Number on the Ttreatment Outcome of Intracytoplasmic Sperm Injection

Objective: To investigate the effect of women's age and the number of retrieved oocytes on embryo quality and clinical outcome after intracytoplasmic sperm injection( ICSI). Methods: A retrospective study was carried out to analyze the clinical data of 520 women receiving ICSI.( 1) According to age,they were divided into less than or equal to 25 years old group( group A),26 to 30 years old group( group B),31 to 34 years old group( group C) and more than or equal to 35 years old group( group D).( 2) According to the oocyte number,they were divided into less than or equal to 4( groupⅠ),5 to 10( group Ⅱ),11 to 15( group Ⅲ),16 to 20( group Ⅳ) and more than or equal to 21( group V). After the period ICSI treatment,abnormal fertilization rate,embryo diapause rate,good quality embryo rate,embryo freezing rate,embryo implantation rate and clinical pregnancy rate between different age group were compared while 2PN cleavage rate,good quality embryo rate,embryo implantation rate,embryo freezing rate,embryo diapause rate and clinical pregnancy rate between different oocyte number group were compared. Results:( 1) Abnormal fertilization rate and embryo diapause rate of group A and group B were lower than those of group C and group D( P 0. 05). Among the four groups,good quality embryo rate and embryo freezing rate showed a decreasing trend from group A to group D( P 0. 05). Embryo implantation rate and clinical pregnancy rate of group A and group B were higher than those of group C and group D( P 0. 05).( 2) 2PN cleavage rate and good quality embryo rate and embryo implantation rate of group Ⅰ were lower than those of the other groups( P 0. 05). Between the five groups,embryo freezing rate showed a decreasing trend from group Ⅰ toⅤ( P 0. 05). Embryo diapause rate of group Ⅰ was higher than that of the other groups( P 0. 05),while clinical pregnancy rate of group Ⅰ was significantly lower than that of the other groups( P 0. 05). Conclusion: With the increase of pregnant women's age,embryo quality is decreased and clinical outcome is poor. When the embryo number is between 11 and 15,embryo quality was high and clinical outcome is good.