Function and metabolism of canine kidneys after aerobic ischemia by orthograde persufflation with gaseous oxygen

In search for new techniques of renal preservation, kidneys were persufflatedin situ with gaseous O2 at a pressure of 90–100 mm Hg via their vascular system (orthograde aerobic ischemia). The results on post-ischemic function and blood flow, and on the changes in the renal tissue levels of the adenine nucleotides were compared to those obtained from kidneys ischemically stored without additional measures (anaerobic ischemia). The kidneys were stored for 4 hrs at 6°C. A contralateral nephrectomy was performed before the preservation period.