Electrophoretic Protein Spectra of Wild-type and Isogenic Monokaryons of Coprinus lagopus

SUMMARY: In their early investigation o the means by which the incompatibility factors of the incompatibility actors of the tetrapolar basidiomycete Schizophyllum commune find physiological or biochemical expression, Raper & Esser (1961) stresse the need to use isogenic strains to minimize differences in proteins other than those attributable to the incompatibility system. Recently Wang & Raper (1969) have shown disc electrophoresis in polyacrylamide gel to be valuable in investigating the protein spectra of various mycelial types of this fungus and have used their results to test the validity of a model for the model of action of the incompatibility factors. In a preliminary investigation of the applicability of disc electrophoresis to the examination of the proteins of the related fungus Coprinus lagopus sensu Buller, the method described was found to give good results. The advantage of using ‘isogenic’ strains of Coprinus is open to question.