Interaction between ipriflavone and other drugs in metabolism in vitro

Objective: To investigate the metabolism-based interaction between ipriflavone(IP) and other drugs in vitro. Methods: IP and propafenone or estradiol,or other drugs were co-incubated with rat liver microsome induced by β-naphthoflavone. The concentrations of residual substrate in microsomal incubates were determined by HPLC. Results: The metabolism of propafenone (10 μg/ml) incubated with 2 μg/ml or 50 μg/ml IP was markedly inhibited compared with control group(P0.01). While the metabolism of 10 μg/ml of estradiol incubated with 10 μg/ml and 100 μg/ml IP showed no marked inhibition. When IP (20 μg/ml) was incubated with 0.5 μg/ml propranolol,0.5 μg/ml propafenone or 5.0 μg/ml estradiol respectively,the metabolic activity of IP was markedly inhibi-ted compared with control group(P0.05,P0.05,P0.02,respectively). Conclusion: The results indicate that there is an interaction between ipriflavone and propafenone during in vitro metabolism,and there might also be cli-nical significance.