Impact of downregulation of NF-κB by activated notch1 on proliferation and apoptosis of cervical carcinoma cells.

Objective To construct the vector of pCLNRX-ICN for expression of recombinant retroviruses amd investigate the impact of overactivation of Notch1 on the growth of Hela cell in cervical cancer patients.Methods The recombinant retroviruses were used to infect HeLa cells.After infected,proliferation of HeLa cells was observed by MTT assay.The growth of HeLa cells in vivo were observed through the nude mice animal model.Apoptosis of HeLa cells were tested using Hoechst 33342 staining and flow cytometry.NF-κB activation and its related genes were detected by EMSA and Western blot.Results HeLa-ICN cells,Notch1 overexpressing cells,were successfully set up.After infection of recombinant retrovirus,the growth of HeLa cells was inhibited significantly.Morever,activated Notch1 induced apoptosis in HeLa cells.Also,up-regulation of IκBα and down-regulation of Bcl-2,c-Myc and cyclin D1,companied by decrease in NF-κB activation,were obseved in HeLa-ICN cells.Conclusion Activated Notch1 could inhibit the growth of cervical carcinoma cells through induction of apoptosis while deduction of NF-κB signaling.