Effects of Unripe Black Raspberry Water Extract on Lipid Metabolism and Oxidative Stress in Mice

Hye Ran Choi, Jung-Hyun Lee, Su Jung Lee, Min Jung Lee, Jong Tae Jeong, and Tae-Bum Lee*Gochang Black Raspberry Research InstituteAbstract We examined the effects of unripe black raspberry water extract (UBR-W) on lipid metabolism and oxidativestress in mice. C57BL/6J mice were divided into 4 groups: those administered a control diet (CTL), high-fat diet (HFD),UBR-W and simvastatin for 12 weeks. In the HFD group, LDL cholesterol were significantly higher than in the CTLgroup. However, the UBR-W treated group showed dose-dependent reduction of plasma LDL levels. Hepatic total lipid,TC, and malondialdehyde were significantly increased in hyperlipidemic mice. However, supplementation with either UBR-W or simvastatin effectively reduced these lipid profiles and lipid peroxidation. UBR-W increased mRNA expression ofthe LDL receptor, sterol regulatory element binding protein 2 (SREBP2), 3-hydroxy-3-methylglutaryl (HMG)-CoAreductase and ATP-binding cassette transporter A1 (ABCA1) compared to that observed in the HFD group. In addition,UBR-W and simvastatin showed significantly reduced oxidized LDL uptake by the scavenger receptor CD36. These resultssuggest that UBR-W is useful for treatment and prevention of hyperlipidemia and lipid peroxidation.Keywords: Unripe black raspberry, High-fat diet, Hyperlipidemia, Lipid metabolism, Oxidative stress