Production of a monoclonal antibody against hemorrhagic activity of Crotalus atrox (western diamondback rattlesnake) venom

Abstract J. C. Perez , V. E. Garcia and S. Y. Huang . Production of a monoclonal antibody against hemorrhagic activity of Crotalus atrox (western diamondback rattlesnake) venom. Toxicon 22, 967 – 973, 1984. — Crotalid venoms have cytotoxic properties which could be useful in medical research. Crotalus atrox venom-hyperimmunized mouse spleen cells were fused with SP2/0 myeloma cells. Forty-one wells containing the hybridoma cells were positive for C. atrox venom, as determined by the enzyme linked immunosorbent assay (ELISA). Cell line 1-e12 was cloned and used to produce ascites tumors in BALB/c mice. The monoclonal antibody produced by cloned and subcultured 1-e12 cells reacted with both C. atrox venom and six other venoms in the ELISA and neutralized the hemorrhagic activity of crude C. atrox venom. A series of monoclonal antibodies could be used in studying the nature of snake venoms.