The Effects of Direct NADPH Oxidase Inhibitor Apocynin on Real-Time Blood Nitric Oxide and Hydrogen Peroxide Release in Femoral Artery/Vein Ischemia and Reperfusion Injury

Conclusions We hypothesize that the femoral I/R vein will show an increase in blood H2O2 release compared to its counterpart sham femoral vein. Whereas, a decrease in NO release is expected in the femoral I/R vein compared to the sham vein of saline control animals. When the direct NADPH oxidase inhibitor, apocynin (T1/2 3h), is given at the beginning of reperfusion we predict a decrease in H2O2 release and an increase in endothelial-derived NO bioavailability compared to saline control group.