Process for the pre-treatment of lignocellulosic biomass by popping method combined with wet milling and process for the production of saccarification and bio-ethanol using the same

PURPOSE: A hydrolysis pre-treatment process for lignocellulosic biomass is provided to enhance the saccharification efficiency of a lignocellulosic biomass and to avoid environmental problems since chemicals are not used. CONSTITUTION: A hydrolysis pre-treatment process for lignocellulosic biomass comprises the steps of: preparing a woody plant-based biomass including pine and oak tree and/or a perennial herb-based biomass including Phragmites communis and purple eulalia; dipping the prepared biomass in water; processing the dipped biomass by microwaves at 600-800W for 10-40 minutes; dehydrating the microwave-processed biomass; and popping the dehydrated biomass under a pressure of 5~30 kgf/cm^2.