Gene Cloning of Deinococcus radiodurans pprM and Its Function Prediction

【Objective】 Through function studies on pprM gene of the radiation-resistance bacteria Deinococcus radiodurans,to lay the foundation for further development and utilization of radiation resistant microbe precious genetic resources.【Method】 Clone the pprM gene by PCR,then transfer it into Escherichia coli though the shuttle expression vector pRADZ3,and its function is analyzed by shock test.【Result】 It is found that pprM gene can improve the salt resistance and drought-resistant ability of E.coli.【Conclusion】 pprM gene is a very important stress-tolerance gene,which has an excellent application prospects in agricultural breeding.