Анализ лёгкой пептидной фракции Лаеннека методами современной протеомики

Laennec preparation is based on standardized human placenta hydrolyzate and is highly effective in the regeneration of tissues. This study presents the results of analysis of the light peptide fraction of Laennec (<3 000 Da) by using three methods: (1) mass spectrometry using Q-Exactive (ThermoScientific, Germany), (2) enzyme immunoassay for the discovery of epitopes of proteins by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies to IGF-1, TGF-1, HGF, VEGF, PDGF, EGF and chemokines (IL-8, IL-1a, IL-1b, TNFa, IL-12) and (3) peptide sequencing. The results indicated high degree of standardization of Laennec concerning the peptide composition. Amino acid sequences of 47 peptides were established using standard software. The composition of Laennec includes the peptide fragments of albumin, collagens Ia2, Va2, XIXa1, «zinc fingers», regenerative growth factors IGF-1, TGF-1, HGF, VEGF, PDGF, EGF. The presence of these peptides in Laennec allowed us to formulate a number of previously unknown molecular mechanisms of the Laennec action