金雀异黄素对II型胶原诱导性关节炎大鼠血清骨保护素及核因子-κB受体活化因子配体的影响

Objective To explore the effects of genistein（Gen） on OPG/RANKL in the serum of Collagen-induced arthritis（CIA） rats. Methods Sixty Sprague-Dawley rats were randomly divided into six groups, with 10 rats in each group respectively, normal group, model group, low-dose Gen treatment group, moderate-dose Gen treatment group, high-dose Gen treatment group and methotrexate group. The levels of OPG and RANKL were determined by ELISA. Results The level of RANKL serum was significantly higher in the model group （0.328±0.044） that in control group（0.328±0.044）, P〈0.05. Moderate-dose Gen treatment group and high-dose Gen treatment group can down-regulate of RANKL（0.144±0.017, 0.163±0.020）respectively. The level of OPG in the model group（0.103±0.013） was the lower than that in the control group（0.268±0.022）, P〈0.05. Gen treatment group and high-dose Gen treatment group can up-regulation of OPG（0.149±0.012, 0.145±0.024）respectively. Conclusion The Gen can enhance the OPG/RANKL ratio for the CIA rats, which may exert their inhibitory effect of RA bone destruction. Key words: Genistein; Collagen induced arthritis; RANKL; OPG