[The effect of extract of ginkgo biloba leaf during the formation of HBV-related hepatocellular carcinoma].

Objective To investigate the effect of extract of ginkgo biloba leaf(EGb) during the formation of HBV-related hepatocellular carcinoma ( HCC ).Methods 99 HBV transgenic mice were randomly divided into control group,high-dose group,low-dose group.High-dose group and low-dose group were intraperitoneal injected 35mg/(kg · d) and 17.5 mg/( kg · d) of the shuxuening injection.Control group without special treatment.The serological markers and immunohistochemical markers in liver tissue will be done at the first 12 months and 18 months.Results ( 1 ) HBV transgenic mice can be found HCC at the 18 months.The incidence of HCC was lower in high-dose group and low-dose group,there was statistically different among the three groups.(2) The semi-quantitative scoring of liver HBx expression was highest in the control group at the 12 months.The semi-quantitative scoring of liver HBx,p53 and Bcl-2 expression was highest in the control group at the 18 months.They all appeared statistically different among the three groups.(3) Spearman correlation analysis showed that HCC incidence and liver tissue HBx,p53,Bcl-2 expression was a certain degree of positive correlation.r was 0.536,0.487 and 0.403,P ＜ 0.05.Conclusion EGb can reduced the incidence of the HCC with HBV transgenic mice.The reason may be that the EGb can reduce liver HBx,p53,Bcl-2 protein expression in the HBV transgenic mice. Key words: Ginkgo biloba; Hepatitis B virus ; Oncogenes; Genes conversion ; Liver neoplosms; Gemes, p53