Tistrella mobilis, halohydrin dehalogenase, gene, vector, recombinant strain and application of halohydrin dehalogenase
2014
The invention discloses a tistrella mobilis, a halohydrin dehalogenase, a gene, a vector, a recombinant strain and application of the halohydrin dehalogenase. The amino acid sequence of the halohydrin dehalogenase is as shown in SEQ ID No: 2; the halohydrin dehalogenase is capable of asymmetrically catalyzing 1,3-dichloropropanol and 1,3-dibromopropanol to generate (S)-epoxy chloropropane and (S)-epoxy bromopropane, and meanwhile, the activity of the halohydrin dehalogenase to (S)-4-chloro-3-hydroxy butyric acid ethyl ester is obviously far higher than that of other halohydrin dehalogenases; as a result, the halohydrin dehalogenase is a natural halohydrin dehalogenase discovered at present having the highest activity to the substrate, and can be applied to preparing epoxides or other chiral epoxides as well as atorvastatin intermediates as a catalyst; besides, the halohydrin dehalogenase is high in catalytic efficiency, excellent in enantioselectivity and high in substrate adaptability, and has brilliant industrial application prospect.
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