DETERMINACIÓN POR PCR EN TIEMPO REAL DE Escherichia coli EN MUESTRAS DE COMIDA RÁPIDA DETERMINATION BY REAL TIME PCR OF Escherichia coli ON PUBLIC FAST FOOD

Using Real Time PCR an microbiology methods we analyzed several food samples expended in streets around Universidad Politecnica Salesiana, Sede El Giron in search for the bacteria Escherichia coli. We selected three fast food restaurants, which were identified as the most important and popular for the community of students from nearby universities. To achieve a reliable statistical analysis were analyzed approximately 15 samples for each food establishment using different products. We developed a protocol for the quantification of samples, using a standard curve for E. coli. Microbiological analyzes were performed by a conventional standard method for cultivation in specific media (EMB, McKonkey) and the real-time PCR with specific primers using the Kit FS