Allele Sizing from Abbott/Celera's FRM1 Triplet Repeat Primed PCR assay

Thirty fragile X samples genotyped previously (ten normal alleles, ten intermediate alleles, and ten premutation alleles) were analyzed in this study. The genotypes ranged from 20-123 CGG repeats. PCR was performed according to the manufacturer’s recommendations. Six of the 30 samples representing two normal alleles, two intermediate alleles, and two premutation alleles were amplified in triplicate and assayed in three separate runs for within run and between run reproducibility studies, respectively. Precision results were used to create bins for automatic allele sizing.