Assessment of Genetic Variations among Medicinal Plant Cassia tora fromDifferent Geographic Regions of Central India Using RAPD Markers
2016
In this study 13 different accessions of medicinal and aromatic plant Cassia tora obtained from different agro
climatic zones of Madhya Pradesh (Central India) were evaluated for its genetic variability using the random amplified
polymorphic DNA (RAPD) markers. During RAPD analysis, amplification of genomic DNA of the 13 accessions by
10 selected primers yielded 88 bright and discernible bands, of which 86 were polymorphic with an average of
9.6 bands per primer and polymorphism obtained was 97.72%. The amplified products varied in size from 2,111 to
153 bp. The Jaccard similarity coefficient value ranged from 0.23 to 0.65 indicate that the distribution of variation is
diverse. A dendrogram constructed based on the UPGMA clustering method revealed three major clusters, which are
again divided into sub clusters. The result revealed rich genetic diversity among Cassia tora accessions obtained
from different agro climatic zones of central India, which gives its suitability to grow and conserve anywhere at any
environmental conditions.
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