VIABILITAS DAN KEUTUHAN MEMBRAN PLASMA SPERMATOZOA EPIDIDIMIS KERBAU BELANG PADA PENAMBAHAN MALTOSA DALAM PENGENCER ANDROMED®: The Viability and Membrane Integrity of Spotted Buffalo Epididymal Sperm in Addition of Maltose into Andromed® Extender

The aim of this study was to investigate the viability and membrane integrity of spotted buffalo epididiymal sperm. Andromed® was used as control extender and the combination of Andromed® with 0.2% (P1) and 0.4 % (P2) maltose were used for the treatments. The results showed that the percentage of motility after thawing in P1 media (46.00 ± 2.00 %) was not significantly different (P>0.05) from P2 (44.00 ± 2.00 %), but there was significantly different (P0.05) from P2 (60.60 ± 1.62 %), but significantly different (P0.05) in the percentage of membrane integrity of spotted buffalo epididymal sperm at the three stages of cryopreservation process in the three different extenders. In conclusion, the addition of maltose into Andromed® could maintain the viability and membrane integrity of spotted buffalo epididymal sperm after thawing. Pustakawan Ludya