Obesity in Saturated Fat Diet Does Not Alter the Proteins Involved in Myocardial Calcium Transit

The aim of this study was to test the hypothesis that obesity by saturated high-fat diet leads to changes in the expression and / or phosphorylation of proteins related to myocardial calcium handling. Wistar rats were divided into two groups: control (C, n = 18; saturated normolipidic diet) and obese (OB; n = 19; saturated high-fat diet) for 30 weeks. Obesity was determined by adiposity index and comorbidities were evaluated. Cardiac morphological profile was estimated by post-death macroscopic analysis. The protein expressions of calcium pump of the sarcoplasmic reticulum (SERCA2a), ryanodine receptor (RYR), calsequestrin (CSQ), phospholamban total (PLB), phospholamban serine 16 (PLB be 16), phospholamban threonine 17 (PLB thr 17), Na+/Ca2+ exchanger (NCX) and L-type calcium channel (L channel) were performed by the method of Western Blot. In this study, the animals showed significant increase in adiposity index (p<0.001) and several comorbidities such as glucose intolerance (p<0.001), hyperleptinemia (p...