Development of FUJI DRI-CHEM v-LIP-P Slide that Has the High Specificity to Pancreatic Lipase

The lipase of blood is mainly a deviation enzyme from pancreas, and its activity is a highly evaluated index for pancreatitis diagnosis in human medical care. However, the sensitivity and specificity for the pancreatitis of the blood lipase activity is low in the veterinarian medical care for dogs and cats , and making rapid diagnosis has been difficult to conduct. The Pancreatic Lipase Immunoreactivity (PLI), a blood diagnosis method, is the most superior in sensitivity and specificity as a pancreatitis marker for the dogs and cats at present. However, because PLI is not easy and rapid, there is a strong need for the development of an easy and rapid method of pancreatitis screening. FUJI DRI-CHEM (FDC), a dry chemistry method, is a system that enables measurement of various enzymic activities in the blood easily and rapidly, which has widely been used in veterinary care. Now we have developed the FDC v-LIP-P Slide having high specificity to measurable pancreas lipase. The measurement principle we used is the triglyceride method. The specificity for the pancreas lipase has also been improved by prescribing colipase, bile salt and sodium dodecyl benzene sulphonate, which is an anionic detergent. In addition, the reactivity has been improved by prescribing triolein, an emulsified substrate, to secure the area of the oil-water interface for the reaction to the pancreatic lipase. It was confirmed that the correlation of FDC v-LIP-P Slide and PLI was excellent. This shows that this slide is a very effective diagnostic product for the rapid screening of pancreatitis.